The assembly is haploid, and the genome is paleotetraploid. It has a haploid genome size of 2400 Mb, and has 10 haploid chromosomes.

Species herbarium records

Herbarium records of Zea mays ssp. huehuetenangensis from AuBuchon-Elder et al 2022 are plotted with black points, The sampled individual this genome was generated from is shown in a colored point. The green background shows the biogeographic ranges of all Andropogoneae species in AuBuchon-Elder et al 2022.

Highlighted Figure 1

Figure 1 from the main text, with Zea mays ssp. huehuetenangensis highlighted with colored points.

Highlighted Figure 2

Figure 2 from the main text, with Zea mays ssp. huehuetenangensis highlighted with colored points.

Dotplot of Zea mays ssp. huehuetenangensis vs. Paspalum vaginatum

Like dotplots shown in Figure 3 of the main text, this shows syntenic blocks in Zea mays ssp. huehuetenangensis along the y-axis, vs P. vaginatum on the x-axis. As Zea mays ssp. huehuetenangensis is a paleotetraploid with a haploid assembly, we expect 2 paths for any x-axis position.

Fractionation of Zea mays ssp. huehuetenangensis

Like plots shown in Figure 4 of the main text, this shows proportion of retained genes in Zea mays ssp. huehuetenangensis along the y-axis, vs P. vaginatum gene index on the x-axis. As Zea mays ssp. huehuetenangensis is a paleotetraploid with a haploid assembly, we expect 2 lines, one for each subgenome.

Highlighted Figure 5

Figure 5 from the main text, with Zea mays ssp. huehuetenangensis highlighted with colored points or lines.

Spatial distribution of genes and TEs

Density of genes and repeats along the longest sequence (contig, scaffold, or chromosome) of Zea mays ssp. huehuetenangensis in 1 Mb bins. Gene annotation shown is all Helixer genes.